1ÊCpermin- ute.Afterwards,dsDNAprobesweredilutedto 10nm in FPassaybuffer(50mM Tris/HCl pH 7.5,100mM NaCl,2mM MgCl2,0.5mM EDTA,10μg/mL BSA,0.1mM DTT, 0.01%Tween- 20)andmixedwith serialdilutionsof peptides1±6 (25μMÐ6 [...] probewasincubatedwith therespectiveexcessof peptidein 1Xassaybuffer(10mM Tris pH 7.5,100mM KCl, 2 mM MgCl2,0.5mM EDTA,10μg/mL BSA,0.1mM DTT, 2.5%glycerol) in afinal volumeof 10μL andincubatedfor 30min at [...] beledantisenseoligonucleotidesweremixedin 1Xannealingbuffer(10mM Tris pH 7.5,100 mM NaCl,1 mM EDTA) to yieldafinal concentrationof 20μM of dsDNA.Subsequently, mixtureswereheatedto 95ÊCfor 10min follo …

IDIOPATHIC PULMONARY FIBROSIS (IPF) is a disease characterized by progressive pulmonary insufficienc

containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 2 mM EDTA, 1% Nonident P-40, 1% sodium deoxycholate, 0.1% sodium dodecylsulfate (SDS), 1 mM phenylmethylsulfonylfluoride, 0.04 TIU/µl aprotinin, 1 mM sodium [...] TGF-β-induced COL1 mRNA expression, WI-38 cells were treated with TGF-β in the presence or absence of 10 µM SP600125, a specific inhibitor of JNK (Han et al., 2001) or 10 µM SB203580, a specific inhibitor [...] amounts of COL1 mRNA were determined by RT-PCR. As shown in Fig. 6A and B, SP600125 inhibited TGF-β-induced COL1 mRNA expression. In addition, SB203580 also suppressed TGF-β-induced COL1 mRNA expression …