Expression of Bradykininereceptors on human HF-15 cells

6.8, 2 mM Bactitracinand collected by centrifugation at 310,000 g for 30 min at 4 °C and used for ligand binding assays. Binding assays were performed by incubation with 5 nM [3H]bradykinin, 2 mM bacitracin [...] 0 12 24 36 48 Time [h] 3 H -b ra dy ki ni n bi nd in g/ m g pr ot ei n 0.5 Gy 2 Gy 5 Gy 10 Gy 0 50 100 150 200 250 0 12 24 36 48 Time [h] 3 H -b ra dy ki ni n bi nd in g/ m g pr ot ei n 0.5 Gy 2 Gy 5 Gy [...] urs (figure 2). 0.5 Gy showed a significant crease, whereas 2 and 10 Gy showed a nificant increase in receptor expression. time point 48 h there was a consistent decrease of B2 receptor expression in cell …