eldorado.tu-dortmund.de/server/api/core/bitstreams/7fa03ed2-5e85-4e6a-a70b-a3c3037194be/content
CONTEXT
coli strain HB101 (F-, thi-1, hsdS20(rB-, mB-), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20(strr), syl-5, mtl-1), strain BL21(DE3) F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr, and [...] coli strain HB101 (F-, thi-1, hsdS20(rB-, mB-), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20(strr), syl-5, mtl-1), strain BL21(DE3) F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr, and [...] volumes of TST buffer (50 mM Tris, 150 mM NaCl, 0.05% Tween 20, pH 7.6) followed by 2 column volumes of 5 mM ammonium acetate, pH 5.0. Bound protein was then eluted with 0.5 M acetic acid, pH 3.4. Fractions …